Open microscopy in challenge based learning

Microscopy is an essential technique in biology, chemistry and physics. Therefore, training in microscopy is crucial in science education. However, there is a gap between theory and practice as microscopes are perceived as “black boxes” rather than instruments that use geometrical and wave optics to provide an image. Active learning methods such as challenge based learning can bridge this gap. We present a challenge based learning course, which uses the open-source microscopy platform UC2 to encourage students to answer a relevant research question while exploring the anatomy of microscopes. The students follow a full research path, in which they start with the design of a microscope, followed by building, calibrating and using it for sample analysis. The challenges we offer are open and versatile, and tailored to the interests of the students. This means, that each group ended up with a different challenge. After two initial lectures about theory, the students started experimenting. A shop was opened for them that contained all the available components, but the students were also allowed to design and print new components with the 3D printers of Innovation Space at the Eindhoven University of Technology. The students obtained very diverse results ranging from tile scans of a full biopsy to moving micromotors in solution. Because of the openness to creativity and the diverse challenges, the students highly appreciate the course. When the students successfully fulfill all the requirements of the course, we believe we give them the tools they will need in their future microscopy projects; from choosing the correct microscopy set-up to scientifically correct processing of the data.